Research Interest

Neurobiology of Olfaction

Understanding the therapeutic potential for modulation of olfactory basal stem cells at circuit level.  As well as elucidating the neural circuitry link essential for the olfactory neurons degeneration. 

Nociception 

Pain is one of the primary responses developed by our body to protect us from harm. However, there are numerous pathological conditions, such as diabetes, viral infections, nerve damage, and inflammation that produce persistent pain. 

Ion channels and neurology

Neurological channelopathies provide an exciting aspect of collaboration between clinicians, molecular geneticists, and cell physiologists to provide a new insights into the biological basis of diseases.

I have working knowledge of TRP's, CaCCs (TMEM16A), KCC2, TREK, and Piezo.

Functional Neurophysiology and Cardiology

From the functional properties of neurons, glia, and brain networks to the disorder of the heart and blood vessels. requires wealth of techniques such as electrophysiology, EEG, ECG, fMRI, calcium imaging, and site-directed mutagenesis to completely understand the etiology and underlying mechanism of diseases.

 

Research Trainings

  • Sr. Postdoctoral research associate, Duke Medical University, USA

  • Postdoctoral research associate, Duke Medical University, USA

  • Postdoctoral research associate, National Institute for Physiological Sciences, Japan

  • Graduate research assistant., National Institute for Physiological Sciences, SOKENDAI Japan 

  • Trainee at University of Cambridge, UK

  • Trainee at Johns Hopkins University School of Medicine, Baltimore MD

  • Invited Researcher, National Institute for Physiological Sciences Japan

  • Bachelor of Science in Biotechnology RTU, India

  • Intern at CSIR-Institute of Genomics and Integrative Biology, India

  • Intern at Indian Academy of Sciences, Bangalore, India

  • Research Assistant at Jaipur National University, India

  • Trainee at Defence Institute of Physiology and Allied Sciences,  Delhi, India 

  • Trainee at Jawaharlal Nehru University, Delhi, India 

2021-present

2017-21

2016-17

2011-16

2014

2012

2010-11

2006-10

2010

2009

2009

2008

2007

Special Skills & laboratory techniques

 

Electrophysiology & Imaging

  • Oocyte-voltage clamp

  • Patch-clamp

  • Calcium Imaging

  • Chloride Imaging

  • Fluorescent Imaging

Applications

  • BLAST

  • FASTA

  • CLUSTALW

  • PDB

  • Adobe

  • PubMed

  • MEGA

  • SnapGene

  • pClamp

  • Prism

  • Origin

  • ImageJ

Cell & Molecular Biology

  • Chimeras b/w species

  • Vector expression

  • DNA extraction

  • Protein extraction

  • Plasmid preparation

  • PCR,

  • Overlap extension PCR,

  • Agarose gel electrophoresis,

  • SDS-PAGE,

  • DH5α transformation,

  • Sequencing,

  • cRNA, transfection

  • Restriction enzyme digests

  • Site directed mutagenesis

  • Western Blotting

  • Immunocytochemistry

  • Immunofluorescent

  • Immunohistochemistry

  • Cell line maintenance (HEK293, Neuro2A, ND7/23, HD10.6, ENB, mOBC, Notch +ve hOBC) 

  • DNA transfection 

  • Electroporation

  • Neuron culture (cortical, DRG, TG)

  • Cryo-sectioning 

Animal handling & Dissections

Frog

  • Feeding & Handling

  • Oocyte Removal surgery

  • Oocyte cRNA injection

Mice

  • Subplantar injection

  • Retro-orbital injections

  • Dorsal Root Ganglia extraction

  • Trigeminal extraction

  • Spinal cord extraction

  • Tissue sectioning

  • Intraperitoneal injection

  • Protein extraction

  • Aortic ring assay

Drosophila

  • Larvae & fly Handling

  • Food preparation

  • Sex-separation

  • 2-choise assay
  • Alcohol assay 
  • Head dissection  

  • Protein extraction

Microbiology skills:

  • Inoculating agar plates.

  • Inoculating broths.

  • serial dilution

  • Using Spectrometer 

  • Gram staining

  • Growth on selective media.

  • Isolating an organism from the environment.

Chemistry skills

  • Solution preparation,

  • Titrations,

  • Osmolarity test,

  • Filtrations,

  • Solubility tests,

  • Freezing point depression,

  • chromatography,